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KMID : 0376619920170040246
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Seoul Journal of Psychiatry
1992 Volume.17 No. 4 p.246 ~ p.258
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Effect of 1-Methyl-4-Phenyl-1, 2, 5, 6, -Tetrahydropyridine on the Muscarinic Cholinergic Receptor in Mouse Brain
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조맹제
우종인/서유헌/박찬웅/이정균
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Abstract
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An investigation into the effect of 1-methyl-4-phenyl-1, 2, 5, 6-tetrahydropyridine (MPTP), a parkinsonism-inducing agent, on the muscarinic cholinergic receptor was done in C57-BL male mouse brain, using [3H]-QNB as ligand. MPTP suppressed
[3H]-QNB
binding with muscarinic receptor in a concentratin-dependent fashion. Scatchardl plot indicated that MPTP was a competitive inhibitor of [3H]-QNB specific binding with nearly the same Bmax and significantly lower Kd values compared with those of
control. Hill coefficients(nH) of control and MPTP (250μM) added group were 1.15±0.127 and 0.56±0.202 respectively. Pretreatment with MAO-B inhibitor, pargyline, before addition of [3H]-QNB binding, but rather a little more strongly displaced
it
than
MPTP or MPP+ alone did. The patterns of displacement of [3H]-QNB by MPTP, MPP+ and pargyline+ MPTP were almost similar to those of well known muscarinic agonists, such as acetylcholine, carbamylcholine and methacholine. These data suggest that
MPTP
might be an effective agonist to muscarinic cholinergic receptor, giving rise to direct influence on the cholinergic neuronal system, independent of dopaminergic system.
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KEYWORD
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